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Description: This substrate produces a magenta chromogen/red fluorescent precipitant in the presence of alkaline phosphatase enzyme. Suitable for brightfield and darkfield visualization in IHC, ICC, and ISH, as well as various blotting applications. Sufficient to produce 120 ml of working solution.
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ImmPACT Vector Red AP Substrate Kit produces a magenta reaction product that is crisper and is 2-4 times more sensitive than the original Vector ® Red substrate kit (SK-5100). It can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.
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The Vector® Red Substrate kit contains all of the reagents (except buffer) necessary to prepare the substrate working solution. These reagents are supplied in convenient dropper bottles. REAGENTS: STORAGE: 4 ml Reagent 1 • Store reagents in original bottles at 2-8 oC. 4 ml Reagent 2 • Avoid storing reagents or working
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Vector DAB Substrate (3,3'-diaminobenzidine) produces a brown reaction product in the presence of peroxidase (HRP) enzyme. This kit also includes nickel chloride, which, when added to the substrate working solution, results in a gray-black reaction product.
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Supplier: Vector Laboratories SK5105. ImmPACT™ Vector® Red AP Substrate Kit produces a bright red reaction product that is crisper and 2-4 times more sensitive than the original VECTOR Red Substrate. Sections stained with ImmPACT Vector Red substrate can be dehydrated, cleared, and permanently mounted. This chromogen can be used with other.
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ImmPACT® Vector® Red Alkaline Phosphatase Substrate the presence of alkaline phosphatase (AP) enzyme. This reaction product is also highly fluorescent and non-fading and can be viewed with Texas RedTM or rhodamine filter systems. Components Preparation of Substrate Working Solution To 5 ml ImmPACT Vector Red Diluent:
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Vector Red substrate kit (SK-5100) does require a Tris buffer with a minimum of 100 mM to be present for a reaction to satisfactorily occur. It is recommended to use a 200 mM Tris buffer at pH 8.2-8.5 for the most optimal staining results. If a buffer with < 100 mM Tris is used to make up the Vector Red working solution it may be that no.
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Vector[R] Red AP Substrate Kit produces a magenta heat stable reaction product that is also fluorescent. Sections stained with the Vector[R] Red substrate can be dehydrated, cleared, and permanently mounted. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.
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ImmPACT™ Vector® Red Substrate The ImmPACT™ Vector® Red Substrate produces a red reaction product in the presence of alkaline phosphatase (AP) enzyme. This reaction product is also highly fluores-cent and non-fading and can be viewed with Texas Red® or rhodamine filter systems.